Simultaneous Determination of 16 Kinds of Mycotoxins in Common Dried Fruits by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry
10.11895/j.issn.0253-3820.170186
- VernacularTitle:超高效液相色谱-串联质谱法同时检测干果中16种真菌毒素
- Author:
Jiao Yu WANG
1
;
Yun Ji NIE
;
Zhen YAN
;
Xia Zhi LI
;
Yang CHENG
;
Nan Xiao ZHANG
Author Information
1. 中国农业科学院果树研究所/农业部果品质量安全风险评估实验室(兴城)
- Keywords:
Mycotoxins;
Ultra high performance liquid chromatography-tandem mass spectrometry;
Dried fruits
- From:
Chinese Journal of Analytical Chemistry
2017;45(10):1556-1563
- CountryChina
- Language:Chinese
-
Abstract:
A method for simultaneous detection of multi-mycotoxins in ten kinds of common dried fruits was been exploited by using ultra performance liquid chromatography-tandem mass spectrometry. The edible part of dried fruits was ground up with certain quantity of water. The mash was extracted by 10 mmol/L citric acid-acetonitrile, purified by C18 column, and filtered using 0. 22-μm organic filtration. All the sixteen mycotoxins were well separated in 8 min on ACQUITY UPLC BEH C18 column with acetonitrile as phase A and 10 mmol/L ammonium acetate solution containing 0. 1% formic acid as mobile phase B. Result showed that the limits of detection ( LODs) for the sixteen mycotoxins were 0. 01-1. 00 μg/L, with linearity range of 1-200 μg/L and correlation coefficients above 0 . 9981 . The average recoveries of the sixteen mycotoxins in ten matrices were 70. 48% -118. 85%, and the relative standard deviation ( RSD, n=6 ) was 0. 3% -11. 9%. This economical, fast, simple and efficient method could be used for simultaneous detection of multi-mycotoxins in different dried fruits matrixes.
