Molecular and Epidemiological Characterization of Carbapenem-Resistant Acinetobacter baumannii in Non-Tertiary Korean Hospitals.
10.3349/ymj.2013.54.1.177
- Author:
Sunok PARK
1
;
Hwa Su KIM
;
Kyeong Min LEE
;
Jung Sik YOO
;
Jae Il YOO
;
Yeong Seon LEE
;
Gyung Tae CHUNG
Author Information
1. Division of Antimicrobial Resistance, Center for Infectious Diseases, Korea National Institute of Health, Cheongwon, Korea. gtchung@nih.go.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Acinetobacter baumannii;
Carbapenem-resistant;
OXA carbapenemase
- MeSH:
Acinetobacter Infections/epidemiology/*microbiology;
Acinetobacter baumannii/classification/*genetics;
Anti-Bacterial Agents/therapeutic use;
Bacterial Typing Techniques;
Carbapenems/*therapeutic use;
DNA, Bacterial/analysis;
*Drug Resistance, Bacterial;
Hospitals;
Humans;
Microbial Sensitivity Tests;
Molecular Epidemiology;
Multilocus Sequence Typing;
Polymerase Chain Reaction;
Prevalence;
Republic of Korea;
beta-Lactamases/genetics
- From:Yonsei Medical Journal
2013;54(1):177-182
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: The increasing prevalence and global spread of carbapenem-resistant Acinetobacter baumannii (A. baumannii) has become a serious problem. The aim of this study was to investigate molecular and epidemiological characteristics of carbapenem-resistant A. baumannii isolates collected from Korean non-tertiary hospitals. MATERIALS AND METHODS: Thirty six non-duplicated carbapenem-resistant A. baumannii isolates were collected from 17 non-tertiary hospitals in Korea between 2004 and 2006. Isolates were typed by multilocus sequence typing and repetitive-sequence-based PCR (rep-PCR). Detection of genes encoding OXA carbapenemase and their relationship with ISAba1 was performed by PCR. RESULTS: Two clones were prevalent among 36 isolates: ST69 (17 isolates, 47.2%) and ST92 (19 isolates, 52.8%). Rep-PCR patterns were diverse and revealed that all isolates were clustered into eight band patterns. The ISAba1-activated blaOXA-23-like and ISAba1-activated blaOXA-51-like genes were prevalent among the carbapenem-resistant A. baumannii isolates. CONCLUSION: The class D beta-lactamase genes of A. baumannii were distributed nationwide in non-tertiary Korean hospitals.
