Effects of exercise on the expression of the LINGO-1 gene in rats after intra-cerebral hemorrhage
10.3760/cma.j.issn.0254-1424.2017.09.001
- VernacularTitle:运动训练对脑出血大鼠LINGO-1表达的影响
- Author:
Shunli LIANG
1
;
You WU
;
Rongbo ZHANG
;
Shuijing ZHANG
;
Bin XU
Author Information
1. 浙江中医药大学附属第二医院(浙江省新华医院)神经内科
- Keywords:
LINGO-1 gene;
Cerebral hemorrhage;
Exercise;
Nerve regeneration
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2017;39(9):641-645
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of exercise on the expression of the LINGO-1 gene after intra cerebral hemorrhage (ICH).Methods Seventy-two Sprague-Dawley rats were randomly divided into a sham operation group,a control group and an exercise training group,each of 24.The three groups were further divided into 1 d,3 d,7 d and 14 d groups.ICH was induced using intra-parenchymal injection of autologous blood.The exercise training group was then forced to exercise on a treadmill.Any recovery of neurological functional was evaluated using Longa scoring,and the expression of LINGO-1 mRNA and protein were detected using the reverse transcription-polymerase chain reaction and western blotting.Results The sham operation group displayed no obvious neurological deficiency,with little expression of LINGO-1 mRNA or protein at any time point.The control group's average Longa score reached a maximum of 3 seven days after the operation,decreasing to 2 after another 7 days.The average expression of LINGO-1 mRNA and protein in that group peaked at 1.335±0.393 three days after the ICH,then decreased to 0.429±0.035 on the 7th day and 0.371±0.038 on the 14th day.In the exercise training group the average Longa score on the 7th day was 2,the average LINGO-1 mRNA level was 0.257±0.042 and the average protein level was 1.142±0.287,all significantly lower than in the control group.Moreover,in the exercise group there was a significant positive correlation between LINGO-1 protein expression and the Longa scores.Conclusion Exercise can decrease the expression of LINGO-1 mRNA and protein and promote the recovery of neural function after ICH.
