The band patterns of Western bolt in different clinical stages of HIV-1 infection
10.3969/j.issn.1672-8467.2017.05.020
- VernacularTitle:HIV-1抗体阳性者WB带型及其在不同临床分期中的特征分析
- Author:
Lin OUYANG
1
,
2
;
复旦大学生命科学学院遗传学研究所 上海 200433
;
jun Tie ZHANG
;
hua Yin ZHANG
;
guang Xiao WANG
;
qiang Guo CHEN
;
Jing WANG
;
zhang Huan ZHU
Author Information
1. 上海市闵行区疾病预防控制中心 上海 201101
2. 复旦大学生命科学学院遗传学研究所 上海 200433
- Keywords:
HIV-1 antibody;
WB band pattern;
CD4+T lymphocyte;
clinical stage
- From:
Fudan University Journal of Medical Sciences
2017;44(5):664-667
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the band pattern characteristics of Western bolt (WB) by analyzingthe change of anti-virus antibodies from patients with different clinical stages of human immunodeficiency virus 1 (HIV-1) infection.Methods Antibodies were detected by WB test,CD4+ T cells by flow cytometer.Results Among 208 cases,193 male cases accounted for 92.79％,and 163 cases of men who have sex with men (MSM) accounted for 78.37％.The antibodies against viral protein gp160,gp120,gp41,p66,p51,p31,p24 showed high positive rates,and no significant different was detected across different clinical stages.The positive rate of p55 antibody,encoding by gag gene,was significantly higher in those HIV positive individuals infected via homosexual compared with HIV-1 positive individuals through heterosexual contact (P＜0.05).The 5 common WB bands patterns were as following:all bands①,missing p55②,missing p39③,missing p55 + p39④ and missing p39 + p55 + p17⑤.Primary stage infection group showed the highest appearance rate of all bands (44.2％).Conclusions The WB bands patterns and CD4+ T lymphocyte counts can help to judge immune status,determine disease stages and monitor disease progression.We should take effective measures for the MSM and floating population.
