Preparation of melanin-based lipid nanobubbles for enhancing multimodal imaging in vitro
10.13929/j.1003-3289.201704096
- VernacularTitle:载黑色素脂质纳泡的制备及体外多模态显像实验研究
- Author:
Liang ZHANG
1
;
Yuanzhi YAO
;
Ke YANG
;
Ying WANG
;
Yang CAO
;
Zhaoxia WANG
;
Zhigang WANG
;
Chongyan LI
;
Dong WANG
Author Information
1. 重庆医科大学附属儿童医院超声科儿童发育疾病研究教育部重点实验室儿童发育重大疾病国家国际科技合作基地儿科学重庆市重点实验室
- Keywords:
Lipid nanobubbles;
Melanin;
Ultrasonography;
Photoacoustic imaging;
Magnetic resonance imaging
- From:
Chinese Journal of Medical Imaging Technology
2017;33(10):1458-1462
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare multi-modality melanin-based lipid nanobubbles (MNBs) contrast agents,and to investigate their manifestations of ultrasound (US),photoacoustic (PA) and MRI in vitro.Methods MNBs and lipid nanobubbles (NBs) were prepared with the method of CHCl3-injection,freeze-drying and C3F8-inflation.The characteristics (shape,grain diameter,loading capacity of melanin and stability) of MNBs were obseved.US,PA and MRI were detected in vitro and the images were quantitatively analyzed.Results MNBs presented with homogenized size distribution,and transmission electron microscope (TEM) images demonstrated that the melanin particles were successfully entrapped into nanobubbles.Meanwhile,the loading capacity of melanin was 90.53 μg/mg.US signal increased in vitro with the rise of MNBs and NBs concentration.The ultrasonic manifestations of MNBs and NBs were the same,and the relative signal enhancement had no significant difference (P＞0.05).With the increased concentration of MNBs,the PA and MRI signals were stronger,but NBs showed no evident enhancement.Conclusion Multi-modality MNBs contrast agents are prepared successfully,which can enhance US,PA and MR imaging.
