Interleukin-6 induces epithelial mesenchymal transition of human peritoneal mesothelial cells by STAT3
10.3760/cma.j.issn.1001-7097.2017.09.011
- VernacularTitle:白细胞介素6通过STAT3诱导腹膜间皮细胞上皮间质转分化
- Author:
Jing XIAO
1
;
Cong WANG
;
Ya'nan GONG
;
Xiaoxiao LI
;
Jie MENG
;
Xiaoyang WANG
;
Xiaoxue ZHANG
;
Yijun DONG
;
Genyang CHENG
Author Information
1. 450000,郑州大学第一附属医院肾脏病医院肾内科
- Keywords:
STAT3 transcription factor;
Interleukin-6;
Cell transdifferentiation;
Peritoneal fibrosis;
Epithelial-mesenchymal transition
- From:
Chinese Journal of Nephrology
2017;33(9):711-717
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of STAT3 transcription factor in IL-6 inducing epithelial mesenchymal transition (EMT) of human peritoneal mesothelial cells (HPMCs).Methods HPMCs were cultured in vitro and grouped.(1) According to the stimulation time with 50 μg/L IL-6,HPMCs were divided into 24,48,72 h groups.(2) HPMCs were grouped 50,100 μg/L according to IL-6 concentration.(3) HPMCs were respectively divided into control group,IL-6 group,empty vector group,empty vector+IL-6 group,virus infecting group and virus infecting+IL-6 group,as lenti-virus vector mediating RNA interference targeting STAT3 was applied.The mRNA expressions of E-cadherin,α-smooth muscle actin (α-SMA) and vascular endothelial growth factor (VEGF) were detected by real time PCR;their protein expressions and the phosphorylation of JAK2 and STAT3 were detected by Western blotting;the expressions and distribution of E-cadherin and α-SMA were observed by immunofluorescence.Results Compared with those in control group,the expression of E-cadherin decreased remarkably (P ＜ 0.05),while the expressions of VEGF and α-SMA and the ratio of phosphorylated (p)-JAK2/JAK2 and p-STAT3/STAT3 increased significantly in IL-6 concentration groups and stimulation time groups (all P ＜ 0.05),which had been dose and time dependent.Compared with empty vector+IL-6 group,virus infecting+IL-6 group had decreased expressions of VEGF and α-SMA,while increased expressions of E-cadherin (all P ＜ 0.05).Conclusions IL-6 can promote VEGF and α-SMA gene expression and prevent E-cadherin gene expression by STAT3,which involves in EMT of peritoneum fibrosis.While STAT3 gene is knocked-down,EMT is inhibited in HPMCs.
