Effects of Dianxianqing Granule on the IL-6 Content and GFAP,Iba-1 Expressions in Hippocampus Tis-sue of Rats with Kainate-induced Epilepsy
10.6039/j.issn.1001-0408.2017.28.11
- VernacularTitle:癫痫清颗粒对海人藻酸致癫痫大鼠海马组织中IL-6含量及GFAP、Iba-1表达的影响
- Author:
Yue QI
1
;
Jitong LI
;
Hong JIANG
;
Guanghan WANG
;
Xiaohu LIU
;
Shaojie XIANG
;
Wenyan QIN
;
Dan WEI
;
Jinghe ZHU
;
Dong JIA
Author Information
1. 辽宁省中医药研究院药理室
- Keywords:
Dianxianqing granule;
Interleukin 6;
Glial fibrillary acidic protein;
Ionized calcium linker molecule 1;
Rats
- From:
China Pharmacy
2017;28(28):3927-3931
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the effects of Dianxianqing granule on the interleukin(IL-6)content and glial fibrillary acid-ic protein(GFAP),ionized calcium linker molecules 1(Iba-1)expressions in hippocampus tissue of rats with kainate-induced epi-lepsy,and explore its mechanism of preventing and treating epilepsy. METHODS:Rats were randomly divided into sham operation group (distilled water),model group (distilled water),phenytoin group (0.03 g/kg,positive control) and Dianxianqing granule low-dose,medium-dose,high-dose groups(4.74,9.47,18.94 g/kg,calculated by crude drug),20 in each group. Rats were intra-gastrically administrated once a day,for 7 d. After 1 h of last administration,except for sham operation group,rats in other groups received single injection of kainite in hippocampus CA1 of left side to induce the epilepsy model. Behavioral changes and death of rats were observed. After 24 h of modeling,enzyme-linked immunosorbent method was used to detect the IL-6 content in hippocam-pus tissue of rats,Nissl staining was used to count the hippocampus neurons,and immunohistochemistry was used to detect the GFAP,Iba-1 expressions in hippocampus tissue of rats. RESULTS:Compared with sham operation group,rats in model group had obvious epilepsy symptoms after modeling,and parts of rats died;IL-6 content and number of neurons in hippocampus tissue were obviously decreased (P<0.01), while GFAP, Iba-1 expressions were obviously enhanced (P<0.01). Compared with model group,epilepsy symptoms and death in each administration group had improved,while IL-6 content in hippocampus tissue were in-creased to varying degrees,with no statistical significance (P>0.05). The numbers of neurons in phenytoin group,Dianxianqinggranule medium-dose, high-dose groups were obviously in-creased(P<0.01);GFAP expression was obviously decreased (P<0.01). Iba-1 expressions in hippocampus tissue in phenyt-oin group,Dianxianqing granule high-dose group were obvi-ously decreased (P<0.01). CONCLUSIONS:Dianxianqing granule can play the role in preventing and treating epilepsy by inhibiting GFAP,Iba-1 expressions in hippocampus tissue and in-creasing the number of neurons in hippocampus tissue.
