Protective effect of N-acetyl-L-tryptophan on intestinal damage after rat hepatic ischemia reperfusion
10.3969/j.issn.1671-8348.2017.27.002
- VernacularTitle:N-乙酰-L-色氨酸对大鼠肝脏缺血再灌注后肠损伤的保护作用
- Author:
Shuhui WANG
1
;
Hongxin JIANG
;
Xiaoshuang LI
;
Mengqi HU
;
Hai YU
;
Ming ZHANG
;
Shuna YU
;
Jianxin WANG
;
Jiying JIANG
Author Information
1. 潍坊医学院临床医学院2013级17班
- Keywords:
ischemia-reperfusion injury;
small intestine;
N-acetyl-L-tryptophan;
apoptosis;
rat
- From:
Chongqing Medicine
2017;46(27):3748-3751
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effects of N-acetyl-L-tryptophan (L-NAT) on intestinal damage after rat hepatic ischemia reperfusion.Methods Twenty-four healthy adult rats were divided into the sham operation group (Sham),ischemia reperfusion group (IR),ischemia reperfusion and N-acetyl-L-tryptophan group(IR+L-NAT).The hepatic ischemia reperfusion model was established by occluding the afferent vessels of the left and middle lobes.The morphological structures of the small intestine were observed by hematoxylin-eosin (HE) staining.The expressions of active caspase-3,Bax and Bcl-2 were detected by immunohistochemistry staining.Results (1) In the IR group,the structure of intestinal villis was destroyed,the intestinal mucosa showed congestion and exfoliation,the epithelial cells had degeneration and necrosis,and infiltration of inflammatory cells appeared;which could be alleviated by L-NAT.(2)The immunohistochemistry showed that compared with the Sham group,the expression of active caspase-3,Bcl-2 and Bax in the IR group was increased,after L-NAT intervention,the Bax and caspase-3 expression was decreased,while the Bcl-2 expression was further increased.Conclusion L-NAT could inhibit the apoptosis of small intestinal epithelial cells caused by liver ischemic reperfusion and attenuates intestinal epithelial damage.
