Effects of propofol on neural stem cells in mouse developing hippocampal dentate gyrus
10.3969/j.issn.1671-8348.2017.27.005
- VernacularTitle:丙泊酚对发育小鼠海马齿状回神经干细胞的影响
- Author:
Sheng JING
1
;
Jing PENG
;
Xiaohang BAO
;
Jie CHEN
;
Zhiyong DU
;
Hong LI
;
He HUANG
Author Information
1. 第三军医大学新桥医院麻醉科
- Keywords:
propofol;
stem cells;
hippocampus
- From:
Chongqing Medicine
2017;46(27):3759-3762,3766
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of propofol on neural stem ceils in mouse developing hippocampal dentate gyrus (DG).Methods Healthy 7 d old mice from the same litters were randomly allocated into three groups:high dose propofol group,low dose propofol group and 10％ fat emulsion control group.All mice were treated with drugs on postnatal 7 d.The mice in high dose propofol group were intraperitoneally injected with 60 mg/kg propofol;the mice in low dose group were intraperitoneally injected 30 mg/kg propofol;while the mice in the control group with equivalent volume of 10％ fat emulsion.Some mice were sacrificed at 24 h after medication injection,and the others were sacrificed at postnatal 14 d.The morphology and expression levels of Ki67,Nestin,BLBP and NeuN in hippocampal DG were detected by immunohistochemical method.Results Healthy 7 d old mice from the same litters were randomly allocated into three groups:high dose propofol group,low dose propofol group and 10％ fat emulsion control group.All mice were treated with drugs on postnatal 7 d.The mice in high dose propofol group were intraperitoneally injected with 60 mg/kg propofol;the mice in low dose group were intraperitoneally injected 30 mg/kg propofol;while the mice in the control group with equivalent volume of 10％ fat emulsion.Some mice were sacrificed at 24 h after medication injection,and the others were sacrificed at postnatal 14 d.The morphology and expression levels of Ki67,Nestin,BLBP and NeuN in hippocampal DG were detected by immunohistochemical method.Conclusion High dose propofol inhibits the proliferation of neural stem cells in hippocampal DG,and impaired the prominence number of neural stem cells and causes neurons dysmaturity.
