Effect of nourishing kidney,activating blood and expelling toxin method Chinese medicine on renal cells apoptosis in CKD rats
10.3969/j.issn.1671-8348.2017.26.004
- VernacularTitle:补肾活血排毒法组方中药对慢性肾脏病大鼠肾脏细胞凋亡的影响
- Author:
Ying LI
1
;
Jia TANG
;
Weijian XIONG
;
Na SONG
;
Zonglang LAI
;
Xin ZHOU
;
Jin ZHONG
Author Information
1. 重庆市中医院肾病科 400021
- Keywords:
chronic kidney disease;
drug therapy;
apoptosis;
the chinese herbs with the function of activating the blood and removing the blood stasis;
Bcl-2/Bax
- From:
Chongqing Medicine
2017;46(26):3611-3613,3616
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the curative effect of nourishing kidney,activating blood and expelling toxin method Chinese medicine on chronic kidney disease (CKD) ratsand influence on cellular apoptosis.Methods The UUO animal model was adopted.SD male rats were divided into the sham operation group,UUO model group,Chinese medicine low dose group and high dose group.The renal function was measured by the automatic biochemical analyzer,the renal histopathologic change was observed by HE staining,the renal tissue apoptosis rate was observed by TUNEL as well as the protein expression levels of Bcl-2 and Bax were detected by Western blot.Results Compared with the UUO model group,the levels of serum creatinine(Scr) and urea nitrogen(BUN) in Chinese medicine various doses groups were significantly decreased(P＜0.05).In the UUO pathologic observation,renal tubular epithelial cells apoptosis,necrosis,exfoliation,inflammatory cells infiltration were found in the model group,the renal damage in the Chinese medicine various doses groups were slighter compared with the UUO group(P＜0.05).The TUNEL method detection showed that the cellular apoptosis number in the UUO group was significantly increased compared with the sham operation group,the Chinese medicine various doses groups could significantly decrease the apoptosis number(P＜0.05).Compared with the UUO model group,the level of Bcl-2 in the Chinese medicine treatment groups was markedly up-regulated,the Bax level was down-regulated,and the ratio of Bcl-2/Bax was increased(P＜0.05).Above results all showed the dose dependent manner.Conclusion The nourishing kidney,activating blood and expelling toxin method Chinese medicine can obviously down-regulate serum Scr and BUN levels,inhibits the renal cells apoptosis in UUO rat and regulates the Bcl-2 and Bax levels.
