Improvement effect of n-3 polyunsaturated fatty acid on rat nonalcoholic fatty liver disease
10.3969/j.issn.1671-8348.2017.26.007
- VernacularTitle:n-3多不饱和脂肪酸对大鼠非酒精性脂肪肝的改善作用
- Author:
Richeng YU
1
;
Jianhua LUO
;
Qian LIU
Author Information
1. 贵州省人民医院内分泌科
- Keywords:
fatty liver,alcoholic;
fatty acids,unsaturated;
n-3 Polyunsaturated fatty acid;
nonalcoholic fatty liver disease;
monocyte chemotactic protein-1
- From:
Chongqing Medicine
2017;46(26):3620-3622
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the improvement effect of n-3 polyunsaturated fatty acid (n-3 PUFA) on rat nonalcoholic fatty liver disease(NAFLD) induced by high fat diet.Methods Thirty Wister male rats were divided into the control group,model group and n-3 PUFA group.The high fat feeding was adopted to establish NAFLD model.After 20 weeks of experiment,7 cases were extracted from each group for detecting serum and liver total cholesterol (TC) and triacylglyceride(TG);other 3 cases were performed the liver HE staining,the levels of MCP-1,iNOS,TNF-α mRNA protein were detected by using the Real time quantitative PCR(qPCR) and Western blot.Results The TC and TG levels in serum and livers of the model group were significantly higher than those in the control group(P＜0.01),but which were evidently decreased after adding n-3 PUFA(P＜0.05).The HE staining clearly observed the rat hepatic cells fatty degeneration in the model group,while polyunsaturated fatty acid had obvious improvement effect on it.The inflammatory molecule MCP-1,iNOS,TNF-α gene expression levels in the model group were significantly higher than those in the control group(P＜0.05),while the expression levels of MCP-1,iNOS and TNF-α in the n-3PUFA group were significantly decreased compared with the model group.Conclusion High fat feeding can cause the severe fatty degeneration in rat liver,but polyunsaturated fatty acid can play obvious improvement effect.
