Targeting effect of TLS9a nucleic acid aptamer on mice hepatic cancer cells
10.3969/j.issn.1671-8348.2017.26.008
- VernacularTitle:TLS9a核酸适配体对小鼠肝癌细胞的靶向作用研究
- Author:
Liping WEI
1
;
Dujin WANG
;
Tiantian ZOU
;
Anxing FEI
Author Information
1. 湖北省黄石市妇幼保健院团城山院区检验科 435000
- Keywords:
liver neoplasms;
nucleic acid aptamer;
TLS9a;
targeted therapy;
mice hepatic cancer cells
- From:
Chongqing Medicine
2017;46(26):3623-3625,3628
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the targeting effect of TLS9a nucleic acid aptamer on mice hepatic cancer cells.Methods The liposome modified with maleimide and loading doxorubicin(DOX) was prepared,then TLS9a nucleic acid aptamer modified by FITC fluorescence and sulfydryl was synthesized,which was coupled to the liposome surface.The entrapment efficiency of DOX was detected by UV spectrophotometry.The dynamic light scattering(DLS) was applied to measure the particle size of nanoparticles and the potential distribution.The uptake of DOX in mice hepatic cancer cells was detected by the Nikon inverted microscope and the mean fluorescence intensity of liposome/DOX and TLS9a-liposome/DOX was detected by flow cytometry.The cells activity was detected by MTT.Results Flow cytometry assay showed that the binding rate of TLS9a nucleic acid aptamer with BNL.1ME.A.7R.1 mice hepatic cancer cells was 54.1％.TLS9a-liposome particle size distribution was in (116.0 ± 5.0)nm.TLS9a-liposome/DOX released DOX quickly at pH 5.0,and the release amount in 72 h was more than 70 ％ of the total release amount.TLS9a-liposome/DOX effectively inhibited the growth of mice hepatic cancer cells BNL.1ME.A.7R.1.Conclusion TLS9a nucleic acid aptamer could specifically combined with mice hepatic cancer cells BNL.1ME.A.7R.1,which could be used to detect mice hepatic cancer cells.
