Study on drug resistance mechanism in carbapenem-resistant Citrobacter freundii and treatment strategies
10.3969/j.issn.1671-8348.2017.26.012
- VernacularTitle:耐碳青霉烯类弗劳地枸橼酸杆菌耐药机制及治疗策略研究
- Author:
Dan XIANG
1
;
Lijun ZHANG
;
Xianyu ZHOU
;
Yunying WANG
Author Information
1. 重庆医科大学附属第二医院检验科
- Keywords:
beta-lactamases;
carbon;
citrobacter,freundii;
carbapenemase;
Citerobacter freundii (C.freundii);
combined chemosensitivity;
resistance mechanisms
- From:
Chongqing Medicine
2017;46(26):3635-3637,3640
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the drug resistance mechanisms of carbapenem-resistant Citrobacter freundii (C.freundii) and its treatment strategies.Methods Clinical data of 17 strains of carbapenem-resistant C.freundii from this hospital were collected.Carbapenemase resistant genes were amplifies by polymerase chain reaction (PCR).The agar dilution method and the broth dilution method were used to determine the minimal inhibition concentration (MIC) of single antimicrobial drug and drug combination,the partial inhibitory concentration index (Σ FICI) was calculated.Results Eight strains were found to produce blaNDM-1 and 9 strains produced blaIMP,blaKPC,blaSPM and blaoxA-48 were not detected in the study.Furthermore,the synergistic effect and addictive effect of fosfomycin combined imipenem accounted for 75.00％,in which,the synergistic effect was up to 56.25 ％.the synergistic effect and addictive effect of fosfomycin and cefoperazone/sulbactam accounted for 50.00％.Conclusion Fosfomycin combined with imipenem or cefoperazone/sulbactam has good antibacterial activity in vitro,but imipenem combined with fosfomycin may have better effect.
