Study on the Mechanism of Anti-myocardial Fibrosis of Salvianolic Acid B
10.6039/j.issn.1001-0408.2017.28.04
- VernacularTitle:丹酚酸B抗心肌纤维化的机制研究
- Author:
Hong LUO
1
;
Chunhua WANG
;
Linglu ZHAO
;
Yu YANG
;
Shiping CHEN
;
Yini XU
;
Hongyu YANG
;
Xiangchun SHEN
Author Information
1. 贵州医科大学贵州省高等学校天然药物药理与成药性评价特色重点实验室
- Keywords:
Salvianolic acid B;
Cardiac fibroblasts;
Type Ⅲ collagen;
Matrix metalloproteinase 9;
Transforming growth factorβ1 signaling pathway
- From:
China Pharmacy
2017;28(28):3900-3903
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the effects of salvianolic acid B(Sal B)on angiotensin Ⅱ(Ang Ⅱ)-induced cardiac fibro-blast proliferation,secretion of type Ⅲ collagen,protein expressions of matrix metalloproteinase 9 (MMP-9),Smad2/3,Smad7, and explore its mechanism of anti-myocardial fibrosis. METHODS:Cells were divided into blank control group(culture medium) Ang Ⅱ model group,Sal B low-dose,medium-dose,high-dose groups (12.5,25,50 μmol/L). After cultured 1 h by blank or drug-containing culture,except for blank control group,cells in other groups were added 1 μmol/L Ang Ⅱ to induce proliferation. for 24 h. MTT method and hematoxylin-eosin staining method were adopted investigate the effect of Sal B on proliferation. Western blot method was adopted to detect the effects of Sal B on protein expressions of type Ⅲ collagen,MMP-9,Smad2/3,Smad7. RE-SULTS:Compared with blank control group,cells in Ang Ⅱ model group were significantly proliferated,protein expressions of type Ⅲ collagen,MMP-9,Smad2/3 were obviously enhanced,protein expression of Smad7 was obviously weakened,with statisti-cal significances(P<0.05). Compared with Ang Ⅱ model group,the cell proliferation in Sal B groups were inhibited,protein ex-pressions of typeⅢcollagen,MMP-9,Smad2/3 were weakened,while protein expression of Smad7 was enhanced. Except the pro-tein expression of type Ⅲ collagen in Sal B low-dose and medium-dose groups,the protein expression of Smad2/3 in Sal B high-dose group did not change significantly,other indexes had statistical significances(P<0.05). CONCLUSIONS:The anti-myo-cardial fibrosis effect of Sal B may be associated with inhibiting the proliferation of cardiac fibroblasts,down-regulating protein ex-pressions of typeⅢcollagen,MMP-9,Smad2/3 and up-regulating protein expression of Smad7.
