Protective role of Gingko Biloba extract in intestinal mucosal barrier of intestinal inflammation mouse
10.3969/j.issn.1671-8348.2017.27.006
- VernacularTitle:银杏叶提取物对肠炎小鼠肠黏膜屏障的保护作用研究
- Author:
Hongwei ZHAO
1
;
Lili HE
;
Hua HAN
;
Yuehong YUE
;
Wei KANG
;
Yeshan ZHU
;
Jianping LIU
Author Information
1. 河北省人民医院消化内科
- Keywords:
EGB 761;
ulcerative colitis;
acute stage animal model;
intestinal mucosal barrier
- From:
Chongqing Medicine
2017;46(27):3763-3766
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective role of Gingko Biloba extract (EGB 761) in intestinal mucosal barrier of intestinal inflammation model mouse.Methods Thirty mice were randomly divided into the control group,model group and EGB 761 group;the animal model was established;the general condition,body mass change,fecal occult blood and colon histopatho1ogical changes were observed,the expressions of occludin-1 proteins in colon tissue were detected by immunofluorescence.Results The body mass in the model group appeared to decrease,drinking water and eating food were significantly decreased compared with that in the control group.However after the EGB 761 intervention,the body mass of the model mice was significantly risen again;little inflammatory cells infiltration could be seen in the EGB 761 group,the inflammatory degree was significantly alleviated compared with the model group.The fluorescence distribution of the claudin-1,occludin and zo-1 in colonic tissues of the model group was more disperse compared with the normal group,the fluorescence intensity was weakened with rough edge;after EGB 761 intervention,the fluorescence in the EGB7 61 group was distributed along with the cellular membrane,the intensity was slightly weakened compared with the normal control group,but was still stronger than that in the model group.Conclusion EGB 761 can improve the inflammatory reaction in mouse colonic tissue,its mechanism may be related with its strengthening the protective effect of intestinal mucosal barrier.
