Protective effect of PEDF on H9C2 myocardial cells under hypoxia condition
10.3969/j.issn.1671-8348.2017.26.002
- VernacularTitle:色素上皮衍生因子对缺氧条件下H9C2心肌细胞保护作用
- Author:
Zhu WANG
1
;
Zhongxin ZHOU
;
Qiuhua GUAN
;
Zhiwei LIU
;
Hongyan DONG
;
Zhongming ZHANG
Author Information
1. 徐州医科大学附属医院心胸外科
- Keywords:
apoptosis;
anoxia;
cardiac;
H9C2 cells;
pigment epithelium derived factor;
dynamin related peptide1;
mitochondrial fission
- From:
Chongqing Medicine
2017;46(26):3605-3607,3610
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effects and possible mechanism of pigment epithelium derived faetor (PEDF) on myocardial cells H9C2 under hypoxia and serum-free condition.Methods H9C2 cells were culture in vitro and performed the hypoxia and serum-free processing.The cells were divided into the control group (H9C2),hypoxia group (hypoxia + H9C2),PEDF group(hypoxia+H9C2 +PEDF) and mitochondrial fission inhibitor(Mdivi-1) group(hypoxia+h9C2+Mivi-1).The apoptotic rate was detected by TUNNEL staining.The proteins levels of dynamin related peptide1 (Drp1) and cleaved-caspase 3 were measured by Western blot.Electron Microscopy and MitoTracker Red were used to detect the mitochondria morphology,the mitochondrial membrane potential was evaluated by cationic dye JC-1.MitoSOXTM was used to detect mitochondrial reactive oxygen species (ROS).Results Hypoxia induced mitochondrial fission(P＜0.05).The hypoxia group (6 h) and control group had statistical difference(P＜0.05).PEDF reduces mitochondrial fission under hypoxia condition(P＜0.05),which had statistical difference between the PEDF group and hypoxia group (6 h)(P＜0.05).PEDF and Mdivi-1 could decrease cell apoptosis under hypoxia condition(24 h),compared with the hypoxia group,the difference was statistically significant(P＜0.05).Conclusion PEDF decrease cell apoptosis by inhibiting H9C2 cells mitochondrial fission under hypoxia condition.
