Bioinformatics analysis for mmu-miR-3475-3P in cardiac development
10.3969/j.issn.1671-8348.2017.26.025
- VernacularTitle:mmu-miR-3475-3P在心脏发育中的生物信息学分析
- Author:
Xing SHEN
1
;
Bo PAN
;
Huiming ZHOU
;
Linjuan LIU
;
Jie TIAN
Author Information
1. 重庆医科大学附属儿童医院心内科
- Keywords:
mmu-miR-3475-3P;
bioinformatics analysis;
target gene;
cardiomyogenesis
- From:
Chongqing Medicine
2017;46(26):3671-3673
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mmu-miR-3475-3P possible participating in biology process and signal pathway.Methods The expression of mmu-miR-3475-3P in the mature mouse heart and in the embryonic mouse heart was measured by qRT-PCR.The target genes were predicted through comprehensively using the common microRNA on-line databases such as TargetScan,miRDB and miRanda,and then the obtained targeted genes were performed the gene function annotation and signal pathway analysis.Results There was significant difference between the expression of mmu-miR-3475-3P in the embryonic mouse heart and the expression in the mature heart.Bioinformatics analysis by using TargetScan,miRDB and MiRanda on miR3475-3P revealed that microRNA was likely to regulate 441 target genes.Conclusion mmu-miR-3475-3P is highly expressed in the embryonic mouse heart.The target genes predicted by mmu-miR-3475-3P are enriched in multiple signal pathways and cellular biological processes.
