Effect of Early Moxibustion Intervention on the Expression of Amyloid β-protein1-40 in 5XFAD Transgenic Mice
10.13460/j.issn.1005-0957.2017.10.1253
- VernacularTitle:灸法早期干预对5XFAD转基因小鼠β淀粉样蛋白1-40表达的影响
- Author:
Jing YU
1
;
hua Ye BAO
;
sheng Yong ZHANG
;
mei Jia CHU
Author Information
1. 杭州市中医院
- Keywords:
Alzheimer disease;
5XFAD transgenic mice;
Wheat-grain-sized moxa cone moxibustion;
Early intervention;
Amyloid β-protein1-40
- From:
Shanghai Journal of Acupuncture and Moxibustion
2017;36(10):1253-1259
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore effect of early moxibustion intervention on cerebral Aβ1-40 in a mouse model of Alzheimer disease (AD) and the mechanism of action of moxibusion in preventing and treating AD.Method Gene phenotype in transgenic AD passage mice was identified using PCR. One and a half-month-old female Tg6799 transgenic mice were randomly allocated, including nine mice to a model group and eight mice to a treatment group. Nine C57BL/6J wild type female mice of the same age and background constituted a normal control group. Wheat-grain-sized moxa cone moxibustion on bilateral points Xinshu(BL15) and Shenshu(BL23) was given to the treatment group. After the completion of treatment, Aβ1-40 expression in mouse frontal cortex and hippocampal region was determined using the immunohistochemical method.Result Aβ1-40 expression in mouse frontal cortex and hippocampal region decreased significantly in the treatment group compared with the model group (P<0.01,P<0.05). Conclusion Early moxibustion intervention can decrease cerebral Aβ1-40 expression and delay AD pathological process in a mouse model of AD.
