Induced apoptosis by quercetin in human colon cancer HT-29 cells through Traf6/TAK1 signaling pathway
10.3760/cma.j.issn.1673-4246.2017.10.012
- VernacularTitle:槲皮素对人结肠癌HT-29细胞凋亡的影响
- Author:
Shi JIANG
1
;
Xingqiang ZHANG
Author Information
1. 445000,湖北省恩施土家族苗族自治州中心医院消化内科
- Keywords:
Apoptosis;
Quercetin;
Human colon cancer cells;
Signaling pathway
- From:
International Journal of Traditional Chinese Medicine
2017;39(10):910-913
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of quercetin on the apoptosis of human colon cancer HT-29 cells and observe the possible mechanism.Methods Human colon cancer HT-29 cells were cultured in vitro and divided into four groups, including the control group, the 16μg/ml quercetin group, the Traf6 inhibitor group and the 16μg/ml quercetin + inhibitor group. The cells in control group were cultured with complete medium and other groups were treated with quercetin or/and Traf6 inhibitor. Flow cytometry was used to observe the impact of quercetin on the apoptosis of HT-29 cells; Western Blot technology was used to detect the expression levels of Traf6, TAK1, p-TAK1, caspase-3, Bax and Bcl-2; RT-PCR was used to investigate the expression level of Traf6 mRNA after treating for 24 h.Results Compared with the 16μg/ml quercetin group, the expression levels of Traf6 (0.59 ± 0.03 vs. 0.96 ± 0.04), p-TAK1 (0.43 ± 0.02vs. 0.72 ± 0.04), caspase-3 (0.59 ± 0.03vs. 0.70 ± 0.04), and Bax (0.48 ± 0.03vs.0.67 ± 0.04) were significantly decreased in 16μg/ml quercetin + inhibitor group(P<0.05). while the expression levels of Bcl-2 (0.54 ± 0.03vs. 0.44 ± 0.02) was significantly increased (P<0.05).Conclusions Quercetin can induce the apoptosis of human colon cancer HT-29 cells and the effective mechanism may relate to the activation of Traf6/TAK1 signaling pathway.
