Activity of Baicalin Against Human Cytomegalovirus In Vitro and Its Effect on the Apoptosis of Human Embryo Lung Fibroblasts Infected with Human Cytomegalovirus
10.3870/j.issn.1004-0781.2017.10.009
- VernacularTitle:黄芩苷体外抗人巨细胞病毒活性及其对感染人胚肺成纤维细胞凋亡的影响
- Author:
Yang LIU
1
;
Yuan HUANG
;
Yi LIAO
;
Wenqing QIN
;
Lingling LIU
;
Ge LI
;
Sainan SHU
;
Feng FANG
Author Information
1. 华中科技大学同济医学院附属同济医院儿科学系
- Keywords:
Baicalin;
Human cytomegalovirus;
Human embryo lung fibroblast;
Apoptosis;
Pro-caspase-3 expression
- From:
Herald of Medicine
2017;36(10):1124-1129
- CountryChina
- Language:Chinese
-
Abstract:
Objective To elucidate activity of baicalin against human cytomegalovirus ( HCMV) in vitro, and explore its effect on apoptosis of human embryo lung fibroblasts ( HEL ) infected with HCMV. Methods CCK-8 kit was used to determine the maximum tolerated dose ( MTC ) of HEL cell to baicalin while the anti-HCMV median efficacious concentration ( EC50 ) of baicalin was determined by standard plaque reduction method. After treatment with baicalin of different concentrations for 24, 48, 72 and 96 h, cell apoptosis and pro-caspase-3 expression was detected by flow cytometry and Western blotting, respectively. Results The MTC of baicalin was 20.6 μg?mL-1; The EC50 of anti-HCMV of baicalin was 16.13 μg?mL-1;The apoptosis rate increased gradually in the groups with low and high multiplicity HCMV infection at early time, showing significant dose-dependent manner. While the ratio of apoptotic cells was going to decrease in high multiplicity infection group 96 h after the infection. The expression of pro-caspase-3 was significantly higher in high-dose baicalin treatment group than in the infection control group ( P<0.05) . Conclusion Baicalin has a direct anti-HCMV effect in vitro. One of the mechanisms might be related with it inhibiting cell apoptosis and antagonizing activation of pro-caspase-3.
