Effect of Electroacupuncture on Cognition and Hippocampal TNF-α and IL-1β Expressions in Rats with Postoperative Cognitive Dysfunction
10.13460/j.issn.1005-0957.2017.09.1116
- VernacularTitle:电针对术后认知功能障碍大鼠认知功能及海马TNF-α和IL-1β表达的影响
- Author:
rong Pei LIU
1
;
Yu ZHANG
;
Min GUI
;
Shu DIAO
Author Information
1. 上海中医药大学附属第七人民医院
- Keywords:
Electroacupuncture;
Cognitive impairment;
Morris water maze;
TNF-α;
IL-1β;
Rat;
Digestive system surgical procedures
- From:
Shanghai Journal of Acupuncture and Moxibustion
2017;36(9):1116-1120
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of electroacupuncture (EA) on the cognition and hippocampal tumor necrosis factor (TNF)-α and interleukin (IL)-1β expressions in rats with postoperative cognitive dysfunction (POCD), and discuss the action mechanism of EA.Method Ninety healthy male senile Sprague-Dawley (SD) rats were randomized into a sham operation group (group C), an operation group (group S) and an EA group (group Z), 30 rats in each group. Each group was then further divided into 3 subgroups: postoperative day 1, postoperative day 3 and postoperative day 7. The Morris water maze was adopted to evaluate the cognitive function, and immunochemistry method was used to detect the changes of TNF-α and IL-1β.Result Compared with group C at the same time points, the latent period was significantly longer and the crossing times were lower in group S on postoperative day 1, day 3 and day 7 (P<0.05), and the differences were most significant on postoperative day 3; compared with group S at the same time points, the latent period was significantly shorter and the crossing times were higher in group Z on postoperative day 1, day 3 and day 7 (P<0.05). Compared with group C at the same time point, the positive expressions of TNF-α and IL-1β were significantly higher in group S (P<0.05); compared with group S at the same time points, the positive expressions were significantly lower in each subgroup of group Z (P<0.05). Conclusion EA can improve the learning and memory of rats after left lateral segmentectomy of the liver, which is possibly related to the inhibition of the over-expressions of TNF-α and IL-1β.
