Expression of miR-93-5p and miR-27a-3p in rectal cancer tissues and its clinical significance
10.3760/cma.j.issn.1004-4221.2017.10.010
- VernacularTitle:miR-93-5p和miR-27a-3p在直肠癌组织中的表达及其意义
- Author:
Yiran HU
1
;
Peng HU
;
Quan WU
;
Wenguang LUO
;
Hongyan ZHANG
Author Information
1. 安徽医科大学附属省立医院肿瘤放疗科
- Keywords:
Rectal cancer;
miR-93-5p;
miR-27a-3p;
Tumor volume;
Carcinoembryonic antigen;
Lymph nodes metastasis
- From:
Chinese Journal of Radiation Oncology
2017;26(10):1156-1161
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the miRNA expression profiles in rectal cancer tissues and their associations with clinical pathological stage, depth of tumor invasion, and lymph node metastasis, and to evaluate the potential of miRNA as diagnostic and prognostic markers of rectal cancer. Methods Human miRNA microarray was used to profile miRNA expression in rectal cancer tissues and matched adjacent normal tissues (n=71). The up-regulated miR-93-5p and down-regulated miR-27a-3p were screened out, and the top differentially expressed miRNA were validated by quantitative real-time polymerase chain reaction ( qRT-PCR) . The relationship between the expression of miRNA and clinical parameters was analyzed by ANOVA and Spearman correlation. Results The expression of miR-27a-3p was down-regulated in miRNA microarray, but was up-regulated in qRT-PCR analysis;the data were relatively discrete. The expression of miR-93-5p was up-regulated in both miRNA microarray and qRT-PCR analysis;the expression level of miR-93-5p in rectal cancer tissues was 3165 times that in adjacent normal tissues ( P=00058);the expression level was correlated with tumor volume ( P= 0004 ) , and was positively correlated with the level of carcinoembryonic antigen ( CEA) before treatment ( P=0001) and the number of lymph nodes metastases (rs=0534, P=0005). Conclusions There is a differential miRNA expression pattern between rectal cancer tissues and matched adjacent normal tissues. The miR-93-5p is highly up-regulated in rectal cancer tissues and may serve as a diagnostic and prognostic marker of rectal cancer.
