Simultaneous determination of eight constituents in Tiantai No.1 Tablets by HPLC-ELSD

VernacularTitle:HPLC-ELSD法同时测定天泰1号片中8种成分
Author: yu Shao LIANG ¹ ; chang Yong ZENG ; mei Xiao TAN ; zhi Zheng WU
Author Information

1. 暨南大学
Keywords: Tiantai No.1 Tablets; chemical constituents; HPLC-ELSD
From: Chinese Traditional Patent Medicine 2017;39(9):1841-1844
CountryChina
Language:Chinese
Abstract: AIM To establish an HPLC-ELSD method for the simultaneous content determination of gastrodin,parishin A,parishin B,ginsenosides Rg1,Re,Rf,Rb1 and Rd in Tiantai No.1 Tablets (Ginseng Radix et Rhizoma,Gastrodiae Rhizoma,Cistanches Herba,etc.).METHODS The analysis of methanol extract of this drug was performed on a 45 ℃C thermostatic Alltima C1scolumn (4.6 mm × 250 mm,5 μm),with the mobile phase comprising of acetonitrie-0.1％ phosphoric acid flowing at 1.0 mL/min in a gradient elution manner.RESULTS Eight constituents showed good linear relationships within their own ranges (r ＞ 0.999 0),whose average recoveries were 96.94％-97.93％ with the RSDs of 1.06％-2.48％.CONCLUSION This simple,accurate and reliable method can be used for the quality control of Tiantai No.1 Tablets.