Simultaneous determination of five constituents in Shanzhuyu Formulated Granules by QAMS
10.3969/j.issn.1001-1528.2017.09.017
- VernacularTitle:一测多评法同时测定山茱萸配方颗粒中5种成分
- Author:
rou Yu TIAN
1
;
mei Jing MA
;
guo Xin WANG
;
shan Jun LI
;
ying Li NIU
Author Information
1. 河北中医学院
- Keywords:
Shanzhuyu Formulated Granules;
gallic acid;
5-hydroxymethylfurfural;
morroniside;
loganin;
cornuside;
QAMS
- From:
Chinese Traditional Patent Medicine
2017;39(9):1845-1849
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish a quantitative analysis of multi-components by single mark (QAMS) method for the simultaneous content determination of five constituents in Shanzhuyu Formulated Granules (Corni Fructus).METHODS The analysis of 80％ methanol extract of this drug was performed on a 30 ℃ thermostatic Wondasil C18column (4.6 mm × 250 mm,5 μm),with the mobile phase comprising of acetonitrile-0.3％ phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 335 nm.With morroniside as an internal standard,the relative correction factors of gallic acid,5-hydroxymethylfurfural,loganin and cornuside were established,followed by the determination of their contents.RESULTS Gallic acid,5-hydroxymethylfurfural,morroniside,loganin and cornuside showed good linear relationships within the ranges of 0.0120-0.120,0.026 8-0.268,0.074 4-0.744,0.058 6-0.586 and 0.0086-0.086 μg (r≥ 0.9999),whose average recoveries (RSDs) were 103.43％ (1.45％),103.36％ (1.50％),104.47％ (0.30％),102.08％(1.74％) and 104.01％ (0.62％),respectively.The results obtained by QAMS approximated those obtained by external standard method.CONCLUSION This stable and reliable method can be used for the quality control of Shanzhuyu Formulated Granules.
