Simultaneous determination of eight flavonol glycosides in Toona sinensis shoots by UPLC
10.3969/j.issn.1001-1528.2017.09.023
- VernacularTitle:UPLC法同时测定香椿芽中8种黄酮醇苷
- Author:
yu Chong GE
1
;
Ling LIN
;
ying Qin GU
;
Nan LI
;
Huan YANG
;
bin Xiao JIA
Author Information
1. 无锡市第三人民医院药学部
- Keywords:
Toona sinensis Roemer;
shoots;
flavonol glycosides;
UPLC
- From:
Chinese Traditional Patent Medicine
2017;39(9):1873-1876
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish a UPLC method for the simultaneous content determination of glycosides,rutinoside,myricitrin,hyperoside,isoquercitrin,guaijaverin,astragalin,quercitrin and afzelin in the shoots of Toona sinensis Roemer.METHODS The analysis of 60％ methanol extract of T.sinensis shoots was performed on a 35 ℃ thermostatic Waters XBridge Shield RP18 column (2.1 mm × 150 mm,1.7 μm),with the mobile phase comprising of acetonitrile-water flowing at 0.35 mL/min in a gradient elution manner,and the detection wavelength was set at 350 nm.RESULTS Eight flavonol glycosides showed good linear relationships within their own ranges (r≥ 0.9992),whose average recoveries were 98.3％-103.5％ with the RSDs of 0.46％-3.36％.CONCLUSION This accurate,stable and reproducible method can be used for the quality control of T.sinensis shoots.
