Quality evaluation of fifteen varieties of Epimedii Folium from different growing areas
10.3969/j.issn.1001-1528.2017.09.027
- VernacularTitle:不同产地15个品种淫羊藿的质量评价
- Author:
han Wei QIN
1
;
Xiang LIU
;
Jun ZHANG
;
hua Chang WANG
;
sheng Yong WEI
;
Yong YANG
Author Information
1. 重庆市中药研究院
- Keywords:
Epimedii Folium;
chemical constituents;
growing areas;
varieties;
UPLC-MS/MS;
principal component analysis
- From:
Chinese Traditional Patent Medicine
2017;39(9):1889-1895
- CountryChina
- Language:Chinese
-
Abstract:
AIM To evaluate the quality of fifteen varieties of Epimedii Folium from different growing areas.METHODS UPLC-MS/MS was adopted in the content determination of icariin,icariside Ⅰ,icariside Ⅱ,epimedin A,epimedin B,epimedin C,sagittatoside A,sagittatoside B,2"-O-rhamnosylicariside Ⅱ and baohuoside Ⅱ.The analysis of 40％ ethanol extract of Epimedii Folium was performed on a 40 ℃ thermostatic Welch C18 column (50 mm × 2.1 mm,3 μm),with the mobile phase comprising of 0.1％ formic acid-acetonitrile flowing at 0.2 mL/min in a gradient elution manner.Then principal component analysis was made.RESULTS Ten constituents showed good linear relationships within their own ranges (r ＞ 0.999 0),whose average recoveries were 97.6％-104.5％ with the RSDs of 1.37％-2.93％.In 46 batches of samples,epimedin C content was the highest,followed by epimedin A content,and icariside Ⅰ content was the lowest.The contents of various constituents showed obvious differences between small-flowered taxa and big-flowered taxa.CONCLUSION This sensitive and rapid method can provide a reference for the development and utilization of Epimedii Folium.
