Influence of Toll-like receptor 5 on biological characteristics of mesenchymal stem cells
10.7644/j.issn.1674-9960.2017.07.003
- VernacularTitle:Toll样受体5对间充质干细胞生物学特性的影响
- Author:
xiao Xiu XIAO
1
;
hua Bing XUE
;
ru Ya QIN
;
feng Hai DUAN
;
hui Shao YE
Author Information
1. 云南农业大学动物科学技术学院
- Keywords:
Toll-like receptor 5;
mesenchymal stem cells;
interleukin;
biological characteristics
- From:Military Medical Sciences
2017;41(7):560-566,571
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Toll-like receptor 5(TLR5) on the biological characteristics of human umbilical cord-derived mesenchymal stem cells (MSCs).Methods The lentiviral vectors expressing TLR5 were constructed and MSCs were infected with related lentiviruses .The overexpression of TLR5 in MSCs was verified by FACS analysis, RT-qPCR and Western blotting before its influence on cell proliferation , surface antigens and cell differentiation was evaluated.The biological function of TLR5 was verified by detecting cytokines IL-6 and IL-8 expression after TLR5 was activated by its agonist CBLB502.Results The results showed that the expression of mRNA and protein of TLR5 was upregulated in MSCs after infection of lentivirus carrying TLR5, but their proliferation , immunophenotypes and differentiation capacity were not influenced . Moreover, IL-6 and IL-8 expression was significantly increased when MSCs-TLR5 were treated with CBLB502 ( P <0.001 ).Conclusion The biological characteristics of MSCs were not affected after infection of lentivirus/TLR5, and the expressed TLR5 was functional in MSCs , which could activate the downstream signaling pathways and then regulate immune response.
