The effect of shRNA interference lentivirus vector targeting rat Sirt1 gene on the expression of Sirt1 in retinal ganglion cell
10.3760/cma.j.issn.1005-1015.2017.05.015
- VernacularTitle:靶向大鼠沉默信息调节因子1基因小干扰RNA慢病毒载体对大鼠视网膜神经节细胞中沉默信息调节因子1表达的影响
- Author:
Ru BAI
1
;
Yue ZHOU
;
Xiaohong LU
;
Jinjin CAI
;
Qing YAO
Author Information
1. 宁夏医科大学基础医学院病原生物学与医学免疫学系
- Keywords:
Silent information regulator proteins;
saccharomyces cerevisiae;
Lentivirus infections;
Retinal ganglion cells;
Cells;
cultured
- From:
Chinese Journal of Ocular Fundus Diseases
2017;33(5):503-507
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of shRNA interference lentivirus vector targeting rat Sirt1 gene on the expression of Sirt1 in retinal ganglion cell (RGC). Methods Four short hairpin (sh) RNA interference sequences targeting rat Sirt1 gene were designed. The target sequences of Oligo DNA were synthesized and annealed to double strand DNA, which was subsequently connected with pGLV3 lentivirus vector to build the lentiviral vector. The positive clones were identified by polymerase chain reaction (PCR) and DNA sequencing. The lentiviral vector construct and lentiviral packaging plasmids were co-transfected into 293T cells, then the titer of lentivirus were determined. The RGC were divided into 6 groups including blank group, negative control group and si-Sirt1-1, si-Sirt1-2, si-Sirt1-3, si-Sirt1-4 groups. Real-time PCR and Western blotting were used to detect the expression of Sirt1 mRNA and protein in the RGC cells. Results PCR and DNA sequencing analysis confirmed that the shRNA sequence was successfully inserted into the lentivirus vector. The concentrated titer of virus suspension was 8×108 TU/ml after the recombinant lentiviral vector successfully transfected and harvested in 293T cells. Comparing with NC group, the expression of Sirt1 mRNA and protein were significantly decreased in the si-Sirt1-1, si-Sirt1-2, si-Sirt1-3 and si-Sirt1-4 groups (F=27.682, 1185.206; P=0.000, 0.000). The si-Sirt1-2 group had the strongest effect in reducing the expression of Sirt1 mRNA and protein. Conclusion The 4 lentiviral vectors harboring RNAi targeting rat Sirt1 gene can effectively down regulate the expression of Sirt1 mRNA and protein in RGC cells.
