MiR-223-3p modulates megakaryocyte polyploidization by targeting MYH10
10.7644/j.issn.1674-9960.2017.07.002
- VernacularTitle:微小RNA-223-3p通过调节MYH10基因促进巨核细胞多倍体化
- Author:
jing Xiao ZOU
1
;
yi Ming QU
;
Fang FANG
;
Zeng FAN
;
Lin CHEN
;
Wen YUE
;
yan Xiao XIE
;
tao Xue PEI
Author Information
1. 南方医科大学
- Keywords:
miR-223-3p;
MYH10;
megakaryocytes;
polymerase chain reaction;
flow cytometry
- From:
Military Medical Sciences
2017;41(7):552-559
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of microRNA-223-3p (miR-223-3p) on megakaryocytic differentiation and maturation, and explore the potential mechanism .Methods The endogenous expression of miR-223-3p during megakaryocyte ( MK) differentiation was detected by real-time PCR.Flow cytometry further indicated that alteration of miR-223-3p in human cell lines exerted effects on MK differentiation and maturation .By performing integrative bioinformatic analysis, the potential miR-223-3p target gene, MYH10,was identified.Real-time PCR, luciferase reporter assay and flow cytometry revealed that MYH10 was a direct target of miR-223-3p.Results Endogenous expression of miR-223-3p was in-creased with the differentiation and maturation of MK .The expression of megakaryocytic surface markers CD41 and CD61 and the ploidy were significantly increased in K562 and Meg-01 cells after transfection with miR-223-3p mimics.The expression of MYH10 decreased with the increase in miR-223-3p.Using a luciferase reporter assay ,we demonstrated that MYH10 was a direct target of MiR-223-3p.Furthermore, direct downregulation of MYH10 promoted MK polyploidization . Conclusion MiR-223-3p might regulate the polyploidization of MK by targeting MYH10.
