ARRDC4 promotes EV71-triggered IL-6 production by enhancing the activation of NF-κB and MAPK signaling pathway
10.7644/j.issn.1674-9960.2017.07.006
- VernacularTitle:ARRDC4通过NF-κB和MAPK信号通路促进EV71诱导的IL-6产生
- Author:
Jun MENG
1
;
qing Ya HE
;
Hong YANG
;
Gang HU
;
quan Jin CHENG
Author Information
1. 华中科技大学
- Keywords:
ARRDC4;
NF-kappa B;
Picornaviridae;
enterovirus A;
human;
interleukin-6;
innate immunity;
macrophages;
RNA;
messenger
- From:
Military Medical Sciences
2017;41(7):576-580
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the role of arrestin domain-containing protein 4( ARRDC4) in regulation of enterovirus 71(EV71) triggered innate IL-6 production and the underlying mechanism .Methods THP-1-derived macrophages (t-M?) were transfected with ARRDC4 specific siRNA and negative control siRNA .The expression and production of IL-6, replication and virus titer of EV71, and the activation of signaling pathway adaptors were analyzed with quantitative real -time PCR, ELISA and Western blot.Results Upon EV71 infection, ARRDC4 was upregulated.ARRDC4 silencing could enhance mRNA expression and production of IL-6, thus increasing the replication and virus titer of EV71.In ARRDC4 silenced t-M?, the activation of p-65,IκBα,ERK,JNK and p38 was promoted.Conclusion ARRDC4 promotes EV71-t riggered IL-6 production by enhancing the activation of NF-κB and MAPK signaling pathway to inhibit EV71 infection, contributing to positive regulation of anti-EV71 innate immune responses .