Effects of rapamycin on promoting oral squamous cancer cell apoptosis by inhibiting TLR4, IL-6 and PGE2 expression
10.3969/j.issn.1671-8348.2017.28.004
- VernacularTitle:雷帕霉素通过抑制TLR4、IL-6、PGE2表达促进口腔鳞状细胞癌细胞凋亡研究
- Author:
Qimei ZHANG
1
;
Cong ZHOU
;
Li YANG
;
Baoqin WU
;
Hong TAN
;
Minhai NIE
Author Information
1. 西南医科大学附属口腔医院牙周黏膜科
- Keywords:
oral squamous cell;
rapamycin;
immune escape
- From:
Chongqing Medicine
2017;46(28):3900-3902
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether the effects of rapamycin on promoting oral squamous cancer cell apoptosis is related with inhibiting TLR4 expression and inflammatory factor IL-6 and PGE2 expression.Methods Human oral squamous carcinoma SCC-15 cell line was cultured and interfered by rapamycin.Then the activity of SCC-15 cells was detected by CCK 8,the SCC-15 cells apoptosis was detected by the Hochest33342/PI double staining,the invasion ability was determined by the transwell method.The TLR4 protein expression level was detected by Western blot and IL-6 and PGE2 expressions were detected by ELISA.Results Rapamycin could promote cell apoptosis,the invasion ability of SCC-15 cells was significantly decreased.After rapamycin intervention,the TLR4 protein expression was decreased and expression levels of IL-6 and PGE2 were reduced,showing statistical difference as compared with the negative group (P＜0.05).Conclusion Rapamycin promotes oral squamous cancer SCC-15 cell apoptosis,which may be related with inhibiting TLR4,IL-6 and PGE2 expression.
