Effect of dehydrocostuslactone on proliferation and apoptosis in hepatic stellate cells

VernacularTitle:去氢木香内酯对人肝星状细胞增殖及凋亡的影响研究
Author: Jian CHENG ¹ ; Bin LIN ; Xiaojie SHEN ; Yan LIU
Author Information

1. 无锡卫生高等职业技术学校
Keywords: dehydrocostuslactone; hepatic stellate cells; cell cycle; apoptosis
From: Chongqing Medicine 2017;46(28):3906-3908,3911
CountryChina
Language:Chinese
Abstract: Objective To investigate the effects of dehydrocostuslactone(Dehy) on human hepatic stellate LX-2 cells proliferation,apoptosis and the possible related mechanisms.Methods After treating the LX-2 cells by different concentrations of Dehy,the MTT assay and flow cytometry were used to assess the influence of Dehy on cell proliferation and cycle distribution of LX-2 cells.The Hoechst 33342 staining and AV-PI dual staining were used to detect the effect of Dehy on cell apoptosis in LX-2 cells.The apoptosis related proteins expression was detected by Western blot.Results After different concentrations of Dehy acting for 48 h could significantly inhibit the proliferation of LX-2 cells,blocked the cellular cycle at stage S and G2/M,meanwhile induced LX-2 cells apoptosis in the concentration dependent manner;Western blot results showed that Dehy could promote the up-regulation of P27 and bax expression and down-regulation of Bcl-2.Conclusion Dehy induces human liver LX-2 cells apoptosis and cell cycle arrest by adjusting the expression of Bax,Bcl-2 and P27.