Detection of Putative T cell Clones Using T cell Receptor beta Chain Gene Clonality Assay in Korean Patients with Aplastic Anemia.
10.3343/kjlm.2009.29.4.269
- Author:
Hyun Jung CHOI
1
;
Myung Geun SHIN
;
Hye Ran KIM
;
Hyeoung Joon KIM
;
Hoon KOOK
;
Seung Jung KEE
;
Soo Hyun KIM
;
Jong Hee SHIN
;
Soon Pal SUH
;
Dong Wook RYANG
Author Information
1. Department of Laboratory Medicine, Chonnam National University Medical School, Chonnam National University Hwasun Hospital, Hwasun, Korea. mgshin@chonnam.ac.kr
- Publication Type:Case Reports ; English Abstract
- Keywords:
Aplastic anemia;
T cell receptor beta chain gene;
T cell clone
- MeSH:
Adolescent;
Adult;
Aged;
Anemia, Aplastic/*diagnosis/genetics/therapy;
Bone Marrow Transplantation;
Female;
Humans;
Male;
Middle Aged;
Reagent Kits, Diagnostic;
Receptors, Antigen, T-Cell, alpha-beta/*genetics;
Republic of Korea;
T-Lymphocytes/*cytology/immunology
- From:The Korean Journal of Laboratory Medicine
2009;29(4):269-276
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: We analyzed T cell receptor beta chain (TCRB) gene to investigate the presence of putative T cell clones and its clinicopathologic implications in Korean patients with aplastic anemia (AA). METHODS: Twenty-nine bone marrow specimens were collected from 20 AA patients, 19 specimens from initial diagnosis and 10 from follow-up. T cell clonality assay was performed using IdentiClone(TM) TCRB Gene Clonality Assay kit (InVivoScirbe Technology, USA) and automatic genetic analyzer. Patients' clinical information and laboratory parameters were also analyzed. RESULTS: Five patients had definitive underlying factors related with aplastic anemia, such as hepatitis B virus (4 cases) and benzene exposure (1 case). Putative T cell clones were detected in bone marrow specimens of 11 (58%) out of 19 patients at diagnosis. The location of putative T cell clones of TCRB gene (diversity region, Dbeta; joining region, Jbeta; variable region, Vbeta) was distributed in Dbeta2+Jbeta2 (6 cases), Dbeta1+Jbeta1 (3 cases), Vbeta+Jbeta1 (2 cases), and Dbeta1+Jbeta2 (2 cases). Interestingly, among seven patients who underwent stem cell transplantation, five patients with no T cell clones detected at diagnosis developed new T cell clones during the follow-up. CONCLUSIONS: Putative pathogenetic T cell clones were detected in most of AA patients in the current study. T cell clonality assay would be useful for investigating the pathophysiology of acquired AA.
