Differential proteomic analysis of renal tissues in patients with IgA nephropathy using quantitative proteomics technique
10.3969/j.issn.1673-4130.2017.20.002
- VernacularTitle:应用定量蛋白质组学技术对IgA肾病肾组织蛋白的鉴定和定量分析
- Author:
Zhenzhen CUI
1
Author Information
1. 广西师范大学生命科学学院
- Keywords:
IgA nephropathy;
isotope labeling;
renal tissue;
GO analysis
- From:
International Journal of Laboratory Medicine
2017;38(20):2804-2807
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze and identify the protein of patients with IgA nephropathy for identification and quantitative a-nalysis using the combination of liquid chromatography and mass spectrometry which combined isobaric tags for relative and abso-lute quantification .Methods Proteomic analysis of renal tissue in patients with IgA nephropathy and normal controls was per-formed to identify different expressed proteins .Results A total of 1860 proteins were identified ,287 proteins were upregulated in renal tissue of IgA nephropathy patients ,287 proteins were downregulated significantly ,finally 9 upregulated proteins and 8 down regulated proteins were identified(protein fold difference greater than 1 .5) .Conclusion Quantitative proteomic technology is effi-ciently applicable for identification and relative quantitation of proteome in renal tissue ,which could get all the information of differ-ent protein .This is useful for us to better understand the relationship of protein and the pathogenesis of IGA nephropathy .
