Terminal Deoxynucleotidyl Transferase Amplification Based DNA-Copper Nanoclusters Sensor for Detection of L-Histidine
10.11895/j.issn.0253-3820.170336
- VernacularTitle:基于末端脱氧核苷酸转移酶扩增DNA-铜纳米簇荧光传感检测L-组氨酸
- Author:
Hui XIAO
1
;
Lin Jing HE
;
Hao XIAO
;
Chan YANG
;
Meng Ze FENG
;
Long Yu YIN
;
Zhong CAO
Author Information
1. 长沙理工大学化学与生物工程学院
- Keywords:
Terminal deoxynucleotidyl transferase;
Poly-thymine;
Copper nanoclusters;
L-Histidine;
Fluorescence analysis
- From:
Chinese Journal of Analytical Chemistry
2017;45(10):1517-1522
- CountryChina
- Language:Chinese
-
Abstract:
A terminal deoxynucleotidyl transferase ( TdT ) amplification based DNA-copper nanoclusters (CuNCs) sensor was developed for detection of L-histidine ( L-His). Single strand DNA containing poly-thymine ( T) sequences were synthesized by TdT in the presence of dTTP. In blank control, poly-T sequences worked as templates of CuNCs due to the affinity between thymine and copper ions( II) . Fluorescence intensity was enhanced when CuNCs formed with reducing agents. In the presence of L-His, the imidazolyl group of L-His worked as a chelating agent that formed L-His-Cu2+ chelated complex. Thus less copper ions were induced in poly-T sequences, and less CuNCs were obtained to produce week fluorescence signals. A good linear correlation was obtained between fluorescence change and the logarithm of the L-His concentration over the range of 5. 0 ×10-9-5. 0 ×10-4 mol/L. The detection limit was estimated as 3. 4 ×10-9 mol/L. And the recoveries were 97. 4%-104. 6% for the actual urine samples. Compared with other methods of synthetic CuNCs, this method allowed to specifically determining L-histidine without template or labeling, which showed good potential in biomedical and clinical analysis.
