Development of Immunochromatographic Strips Based on Covalently Conjugated BHHCT-Eu3+@SiO2 for Rapid and Quantitative Detection of Kanamycin
10.11895/j.issn.0253-3820.170015
- VernacularTitle:基于BHHCT-Eu3+@SiO2荧光稀土二氧化硅纳米颗粒的免疫层析试纸条检测卡那霉素
- Author:
Jie Bing ZHAO
1
;
Bao Jin ZHAO
;
Hua Xiao QI
;
Qiang Ming ZOU
;
Yan CHEN
;
Fan ZHANG
;
Yi YANG
Author Information
1. 北京化工大学理学院
- Keywords:
Kanamycin;
Microwave-assisted;
EuropiumⅡ chelate-bonded silica nanoparticles;
Fluorescence lateral flow immunoassay;
Milk
- From:
Chinese Journal of Analytical Chemistry
2017;45(10):1467-1474
- CountryChina
- Language:Chinese
-
Abstract:
As a kind of fluorescent nanoprobe, BHHCT-Eu3+ @ SiO2 fluorescent nanoparticles were synthesized using microwave irradiation. Transmission electron microscopy characterization showed that the nanoparticles were in spherical shape with particle size of about 36 nm. The BHHCT-Eu3+@SiO2 exhibited good fluorescence property, with a maximal excitation wavelength of 343 nm and an maximal emission wavelength of 615 nm. The fluorescence lateral flow immunoassay ( LFIA ) was established for rapid and quantitative detection of kanamycin ( Kana) in milk after BHHCT-Eu3+@SiO2 fluorescent nanoparticles were conjugated with Kana antibody, with dextran as a linker. The limit of detection of Kana with the LFIA method was 0. 85 ng/mL with IC50 of 12. 76 ng/mL, and the detection range was from 3. 0 ng/m to 76 ng/mL. The recoveries of Kana in milk were between 93 . 7% and 97 . 4% with RSD of 3 . 1%-4 . 6%, and cross-reactivity of the fluorescence immunoassay strip for Kana determination was<1%. The detection results of kana in milk samples between the LFIA and traditional ELISA method showed good correlation.
