Anti-inflammatory effects of Daphniphyllum calycinum extract
10.3969/j.issn.1001-1528.2017.09.003
- VernacularTitle:牛耳枫提取物的抗炎作用
- Author:
Qing SONG
1
;
xia Fen ZHU
;
yu Dong LI
;
Hao ZHANG
;
quan Jia CHEN
Author Information
1. 西安交通大学第一附属医院妇产科
- Keywords:
Daphniphyllum calycinum Benth;
anti-inflammatory;
lipopolysaccharide;
RAW264.7 macrophage;
endotoxemia
- From:
Chinese Traditional Patent Medicine
2017;39(9):1771-1776
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the in vitro and in vivo anti-inflammatory effects of extract from Daphniphyllum calycinum Benth and its mechanism of action.METHODS LPS-induced mouse RAW264.7 macrophage and mouse endotoxemia model were used to observe the influences of D.calycinum extract on the levels of NO,TNF-α,IL-1β and IL-10.The toxicity of D.calycinum extract on RAW264.7 macrophage was determined by MTT assay.Fifty female BALB/c mice were administered with D.calycinum extract by gavage.Fourteen days later,the mice were intraperitoneally injected with LPS,the levels of NO,TNF-α,IL-1 β and IL-10 in serum were determined by Griess and ELISA kit.The protein expressions of NO and TNF-α in mouse liver were studied by immunohistochemistry method.RESULTS D.calycinum extract could significantly inhibit the release of NO,TNF-α,IL-1 β and IL-10.Immunohistochemistry results showed that the extract could inhibit the protein expressions of iNOS and TNF-α.CONCLUSION D.calycinum extract has a significant anti-inflammatory effect,and its mechanism may be related to decreasing the release of inflammatory factors.
