Simultaneous determination of four constituents in Shugan Pills by HPLC

VernacularTitle:HPLC法同时测定舒肝丸中4种成分
Author: zhu Hui SUN ¹ ; lei Xiao WANG ; Le XU ; li Yong LIU
Author Information

1. 河北省药品检验研究院
Keywords: Shugan Pills; paeoniflorin; naringin; hesperidin; neohesperidin; HPLC
From: Chinese Traditional Patent Medicine 2017;39(9):1850-1854
CountryChina
Language:Chinese
Abstract: AIM To establish an HPLC method for the simultaneous content determination of four constituents in Shugan Pills (Toosendan Fructus,Corydalis Rhizoma,Paeoniae Radix Alba,etc.).METHODS The analysis of methanol extract of this drug was performed on a 35 ℃ thermostatic C18 column (250 mm ×4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-0.05％ phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 230 nm.RESULTS Paeoniflorin,naringin,hesperidin and neohesperidin showed good linear relationships within the ranges of 0.040 44-1.011,0.045 20-1.130,0.047 08-1.177 and 0.047 24-1.181 μg (r =0.999 9),whose average recoveries were 100.5％,98.6％,98.4％ and 101.3％ with the RSDs of 1.5％,0.9％,1.2％ and 1.1％,respectively.The contents of various constituents in the samples with three dosage forms from seven manufacturers showed obvious differences.CONCLUSION We should pay attention to the uneven quality of Shugan Pills.