Establishment of HPLC fingerprints of Zhihuang Tongfeng Decoction and determination of four constituents
10.3969/j.issn.1001-1528.2017.09.019
- VernacularTitle:知黄痛风汤HPLC指纹图谱建立及4种成分测定
- Author:
ling Meng HUANG
1
;
Xiong WANG
;
li Ji ZOU
;
Jun WU
;
qiao Qing YIN
;
hu Jin WU
;
you Xiang LI
Author Information
1. 湖北中医药大学药学院
- Keywords:
Zhihuang Tongfeng Decoction;
HPLC fingerprints;
berberine hydrochloride;
mangiferin;
geniposidic acid;
salvianolic acid B
- From:
Chinese Traditional Patent Medicine
2017;39(9):1855-1860
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish HPLC fingerprints of Zhihuang Tongfeng Decoction (Phellodendri chinensis Cortex,Anemarrhenae Rhizoma,Plantaginis Semen,etc.) and to determine the contents of four constituents.METHODS The analysis of aqueous extract of this drug was performed on a 30 ℃ thermostatic Wondasil C18 column (4.6 mm ×250 mm,5 μm),with the mobile phase comprising of acetonitrile-0.5％ acetic acid in a gradient elution manner,and the detection wavelength was set at 245 nm.RESULTS There were sixteen common peaks in the HPLC fingerprints of ten batches of samples with the similarities of more than 0.9.Berberine hydrochloride,mangiferin,geniposidic acid and salvianolic acid B showed good linear relationships within the ranges of 0.636 2-3.575 μg (R2 =0.9999),0.338-1.425 μg (R2 =0.9990),0.6452-2.7188 μg (R2 =1) and0.938 8-5.275 μg (R2 =0.999 3),whose average recoveries were 100.56％,98.35％,100.25％ and 102.11％ with the RSDs of 2.41％,1.17％,1.24％ and 2.02％,respectively.CONCLUSION This accurate,reliable and specific method can be used for the quality control of Zhihuang Tongfeng Decoction.
