Salvianolic acid b improved endothelial function through activating ampk in diabetic mice
10.3969/j.issn.1006-5725.2017.20.009
- VernacularTitle:丹参酚酸B上调AMPK改善糖尿病小鼠血管内皮功能
- Author:
Jixin HOU
1
;
Dan WANG
;
Jingyu KAN
;
Jindong WAN
;
Sen LIU
;
Fang WANG
;
Jiaxin LIU
;
Mingqing HUANG
;
Peijian WANG
Author Information
1. 610500,成都医学院第一附属医院心血管内科
- Keywords:
diabetes;
salvianolic acid B;
endothelial function;
oxidative stress;
adenosine 5′-mo-nophosphate-activated protein kinase
- From:
The Journal of Practical Medicine
2017;33(20):3367-3371
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Salvianolic Acid B(Sal B)on vascular function of db/db mice and reveal the potential mechanism. Methods 20 male db/db mice were divided into 2 groups,the con-trol group(n=10)and Sal B group(n=10). 10 age-matched male C57BL/KsJ mice were used as the wild type control. Mice in Sal B group were given Sal B ,100 mg/(kg · d)by tube. Mice in db/db control group and in wild type control were given the same volume of saline. Body weight,tail blood pressure,heart rate and fasting blood glucose level were measured every week. After 6-weeks treatment ,thoracic aorta was obtained and used to detect the levels ofsuperoxide anion and NO,vascular function,eNOS,p-eNOS,AMPK and p-AMPK. Results Sal B could reduce the body weight and fasting blood glucose level of db/db mice ,but had no effect on blood pressure. Sal B could decrease the level of superoxide inon,increased NO level,and improved endothelium-dependent but not endothelium- independent diastolic function. Sal B could increase phosphorylation levels of eNOS and AMPK. Conclusion Sal B can reduce the oxidative stress ,increases NO level in vasculature ,and improves the endo-thelium-dependent vasodilation in the diabetic mice ,which may be associated with the promotion of AMPK phos-phorylation.
