Effect of PM2.5 exposure on in vitro fertilization (IVF) and embryo development in mice
10.3969/j.issn.1006-5725.2017.20.010
- VernacularTitle:PM2.5暴露对小鼠体外受精及胚胎发育的影响
- Author:
Qiuhua LI
1
;
Xiaoli ZHU
;
Sida WEI
;
Zheng WU
;
Zehu ZHAN
;
Ge SONG
Author Information
1. 广东省计划生育科学技术研究所
- Keywords:
PM2.5;
in vitro fertilization;
egg quality;
high-quality embryos;
TNF-α;
IFN-γ
- From:
The Journal of Practical Medicine
2017;33(20):3372-3375
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of PM2.5 exposure on the quality of IVF eggs and the quality of embryos. Methods 40 healthy female Kunming mice and 10 male mice were used in this study. 40 female mice were randomly divided into 4 groups:low dose of PM2.5 group (1.5 mg/kg),middle dose of PM2.5 group (7.5 mg/kg),high dose of PM2.5 group (15 mg/kg) and the control group (to give the same volume of saline),with 10 mice in each group. PM2.5 was administered via tracheal instillation. The number of oocytes ,fertilization rate ,abnormal fertilization rate ,2PN cleavage rate ,2PN blastocyst rate and blastocyst formation rate were determined ,as well as levels of TNF-α and IFN-γ. Results The number of oocytes , fertilization rate and cleavage rate of 2PN were significantly higher in PM2.5 high dose of PM2.5 group than those in PM2.5 group. The rate of high quality embryos and blastocyviast formation were decreased ,but abnormal fertilization rate were decreased (P < 0.05). The expressions of TNF-α and IFN-γ in the high-dose of PM2.5 group were significantly higher than those in the middle-dose of PM2.5 group and in the low-dose of PM2.5 group(P < 0.05),while the expressions of TNF-α and IFN-γ in the middle dose group was significantly higher than those in the low dose group(P<0.05). Conclusions PM2.5 exposure can decrease the number of oocytes , fertilization rate ,2PN cleavage rate ,2PN blastocyst rate and blastocyst formation rate of in vitro fertilization in mice.
