Mechanism study of the role of rhuPAa-melittin in ovarian cancer cells in vitro and in vivo

VernacularTitle:rhuPAa-melittin在卵巢癌体内外的作用机制
Author: Shiguang JIN ¹ ; Yan DAI ; Cheng LI ; Changxi LI ; Daxin WANG
Author Information

1. 江苏省苏北人民医院医学实验研究中心
Keywords: rhuPAa-melittin; ovarian cancer cells; flow cytometery; transplant tumor
From: The Journal of Practical Medicine 2017;33(19):3173-3176
CountryChina
Language:Chinese
Abstract: Objective To investigate the role of the activated rhuPAa-melittin in ovarian cancer cells and to study the inhibitory effect of rhuPAa-melittinon on ovarian cancer cells. Methods rhuPAa-melittin was used to treat the ovarian cancer cells at different concentrations for 48 hrs. Then flow cytometery was applied to detect the cell cycle and cell apoptosis. rhuPAa-melittin protein was delivered to the mouse mode to investigate the effect of rhuPAa-melittin on the growth of the xenotransplanted tumor. Results rhuPAa-melittin was used to treat ovarian cancer cells at the concentration of 0 ,4 ,8 and 16 μg/mL for 48 hrs ,respectively. The results of cell apoptosis assay was 1.16%,3.83%,6.51% and 10.2%,respectively. Moreover,different concentrations of rhuPAa-melittin had no effects on the cells at G0/G1 phase,rhuPAa-melittin inhibited S phase cells to process into G2/M phase, contributing to suppressing the growth of ovarian cancer cells. Conclusion The in vivo and in vitro studies revealed that rhuPAa-melittin inhibits the growth of ovarian cancer.