The experimental research of microRNA-96-5p downregulating mTOR expression of BGC-823 cells
10.3969/j.issn.1006-5725.2017.18.004
- VernacularTitle:microRNA-96-5p对胃癌细胞哺乳动物雷帕霉素靶蛋白表达的影响
- Author:
Zhaoyan YU
1
;
Jie DING
;
Ping YUAN
;
Zhongmin ZHANG
Author Information
1. 贵州省人民医院普外科
- Keywords:
gastric cancer;
microRNA-96-5p;
mammalian target of rapamycin
- From:
The Journal of Practical Medicine
2017;33(18):2991-2994
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of microRNA-96-5p(miR-96-5p)on expression of mam-malian target of rapamycin(mTOR)in gastric cancer cells. Methods Lentiviral vector,LV-hsa-mir-96-5p was transfected into BGC-823 gastric cancer cells. The expression of miR-96-5p and mTOR mRNA after transfection was detected by qRT-PCR,and the expression of mTOR protein was detected by Western blot. Results At 72 h after transfection,the expression level of miR-96-5p in the experimental group was 20.4 times of that in the blank control group(P<0.05),while the expression level of mTOR gene was downregulated to 0.47 times of that in the blank control group (P < 0.05),and mTOR protein relative expression was significantly reduced compared with the control group (P < 0.05). Conclusion Overexpression of miR-96-5p can downregulate the expression of mRNA and protein of mTOR gene in gastric cancer cells,thus,mTOR may be one of the downstream target genes of miR-96-5p.
