Effect of transplantation of human amniotic mesenchymal stem cells on the lung tissue pathologic change in paraquat induced pulmonary fibrosis rats
10.3969/j.issn.1006-5725.2017.18.006
- VernacularTitle:羊膜间充质干细胞移植改善百草枯中毒肺纤维化大鼠肺组织病理学变化
- Author:
Yuxiang LI
1
;
Fang HE
;
Yuying WANG
;
Shangfu XU
;
Zulin LIU
;
Xue WAN
;
Limei YU
Author Information
1. 遵义医学院附属医院/贵州省细胞工程重点实验室/贵州省生物治疗人才基地
- Keywords:
pulmonary fibrosis;
paraquat;
histopathology;
specifical antibody of anti-human cell nucleus
- From:
The Journal of Practical Medicine
2017;33(18):2999-3003
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the impact of transplantation of human amniotic mesenchymal stem cells(hAMSCs)on the histopathological change in paraquat-induced pulmonary fibrosis in rats. Methods Forty-six female SD rats were randomly divided into the sham surgery group and the hAMSCs transplant group. Pulmonary fibrosis model was induced by 2％ of paraquat intragastric administration(100 mg/kg/rat). hAMSCs were injected through caudal vein(2 × 106 cells/mL/rat). The histopathological changes were observed through microscopy after HE and the immunohistochemical staining. Results General conditions in rats received hAMSCs transplantation were better than those of the model rats. More large area and white fibrosis nidus were observed in bilateral lung of model rats,with less dispersal spot or nidus. The construction of lung tissue was disordered in the model rats. The thickness of alveolar wall was found increased. There were large area interstitial hyperplasia and a large number of inflammatory cells infiltrations. The construction of lung tissue was apparently improved. A majority of alveolar wall was monolayer cell. There were only less and small area with interstitial hyperplasia. Inflammatory cell infiltration was significantly decreased. The anti-human nucleus specific antibody positive hAMSCs were observed planted and survived in lung interstitial tissue. And few hAMSCs were observed planted in alveolar wall. Conclusion The transplanted hAMSCs can be planted and survived in lung tissue ,and may play a therapeutic role in araquat-induced pulmonary fibrosis.
