Expression of miR-34a in the myocardium of mice received Danggui Buxue Decoction therapy following myocardial infarction
10.3969/j.issn.1006-5725.2017.18.008
- VernacularTitle:miR-34a在当归补血汤抗小鼠心肌梗死后心室重塑中的表达
- Author:
Zhiwen FAN
1
;
Yiqiao ZHAO
;
Long LING
;
Yusheng XI
;
Wen JIN
;
Bufan HE
;
jie Wei ZEN
Author Information
1. 中国人民解放军第421医院心内科
- Keywords:
ventricular remodeling;
danggui buxue soup;
miR-34a;
Sirt1
- From:
The Journal of Practical Medicine
2017;33(18):3009-3012
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Danggui buxue decoction on ventricular remodeling and miR-34a expression after myocardial infarction in mice. Methods Mice myocardial infarction model were estab-lished by the left anterior descending coronary artery embolization. Then the mice were divided into Danggui buxue Decoction group and model group. Mice in Danggui buxue decoction group were given Danggui buxue decoction , and mice in the model group were given the equal volume of saline for 8 weeks. Cardiomyocyte apoptosis was detect-ed by TUNEL staining. Myocardial fibrosis was detected by Masson staining. The expression of miR-34a was detect-ed by quantitative PCR and the expression of Sirt1 was detected by Western blot assay. Results Compared with the model group ,the EF value of the mice was significantly improved (P < 0.05),the myocardial cell index decreased(P<0.05),the content of collagen fibers decreased(P<0.05),the expression of miR-34a decreased, but the expression of Sirt1 increased(P<0.05)in Danggui buxue Decoction group. Conclusion Danggui buxue decoction can effectively inhibit ventricular remodeling of mice following myocardial infarction ,which is associated with the suppression of miR-34a and the increase of Sirt1.
