Effect of ozone combined with arthroscopy on expression of IL1R I,CXCL13 and IL24 gene in synovium of osteoarthritis of knee joint
10.3969/j.issn.1006-5725.2017.18.019
- VernacularTitle:臭氧联合关节镜对膝关节骨关节炎滑膜IL1RⅠ、CXCL13与IL24的影响
- Author:
Naxin FU
1
;
Chao MA
;
Jin SUN
;
Hui HUANG
;
Wei ZHAO
;
Zhenzhen MENG
;
Li TIAN
;
Zhaoqi LIU
Author Information
1. 三峡大学第三临床医学院葛洲坝集团中心医院
- Keywords:
ozone;
arthroscopy;
osteoarthritis;
IL1RI;
CXCL13;
IL24
- From:
The Journal of Practical Medicine
2017;33(18):3055-3059
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of medical ozone on the expression of IL1R,CXCL-13 and IL24 gene in synovial membrane of osteoarthritis of knee joint. Methods Sixty-five cases of knee osteoarthritis including 11 lost-cases were selected ,and randomly divided into combination group and arthroscopy group with 27 cases in each group. After arthroscopic surgery ,combination group performed intra-articular injection of 40μg/mL concentration of medical ozone 40 mL/week for 2 weeks but arthroscopic surgery group had no ozone injection. Differences of the expression of IL1R I,CXCL13 and IL24 gene and protein in synovium were compared before and after the treatment in two groups by RT-PCR and Western blot. Results Expression of IL1R I gene and protein in synovium of combination group was significantly lower than that of arthroscopy group and it showed statistical significance(P<0.01). Expression of CXCL13 gene and IL24 gene and protein in synovium of combination group was higher than those of arthroscopy group and it had statistical significance (P < 0.05). Conclusions Medical ozone can reduce the symptoms of arthritis and slow synovitis progress through influencing the expression of IL1R, CXCL-13 and IL24 gene. The effect of ozone combined with arthroscopic is better than that of simple arthroscopic debridement,but cannot stop and reverse the progression of the disease completely.
