The role of MiR-155 in the pathogenesis of diabetic nephropathy by regulation of Smad5
10.3969/j.issn.1006-5725.2017.20.003
- VernacularTitle:MiR-155调控Smad5促进糖尿病肾病肾脏纤维化作用
- Author:
Changjie GUAN
1
;
Feng HE
;
Shanshan ZHOU
;
Jun HUANG
;
Haoxiong CHEN
;
Riguang LIU
;
Junzhou FU
Author Information
1. 510180,广州市第一人民医院肾内科
- Keywords:
diabetic nephropathy;
MiR-155;
Smad5;
renal fibrosis
- From:
The Journal of Practical Medicine
2017;33(20):3340-3344
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of miR-155 in diabetic nephropathy(DN)and its mecha-nism. Methods MiR-155 expression level in kidney was detected by real-time PCR and in situ hybridization. The target gene of miR-155 was predicted by bioinformatics and verified by Western Blot and double luciferase reporter activity. Western Blot was used to detect the related marker proteins of mesangial cells proliferation and mesangial matrix. Results (1)The expression of miR-155 increased in DN renal tissue and high glucose-stimulated renal cells.(2)MiR-155 was related to the regulation of Smad5 gene expression.(3)MiR-155 promoted the mesangial cells proliferation and increased extracellular matrix by down-regulating Smad5 expression. Conclusions MiR-155 can promote the mesangial cells proliferation and renal fibrosis by regulating Smad5 gene,providing a basis for further understanding the pathogenesis of DN.
