Anti-Inflammatory Effect of Near-Infrared Irradiated Cell Culture Media.
10.3343/kjlm.2009.29.4.338
- Author:
Sang Gyung KIM
1
;
Im Hee SHIN
;
Chang Hyuk CHOI
;
Jung Yoon CHOE
Author Information
1. Department of Laboratory Medicine, Catholic University of Daegu, School of Medicine, Daegu, Korea. sgkim@cu.ac.kr
- Publication Type:Original Article ; English Abstract
- Keywords:
Near-infrared (NIR);
Anti-inflammatory mediator;
IL-1 beta;
Nitric oxide (NO);
Inducible nitric oxide synthase (iNOS)
- MeSH:
Animals;
Anti-Inflammatory Agents/*chemistry;
Cattle;
Cell Line;
*Culture Media;
Cyclooxygenase 2/metabolism;
Humans;
*Infrared Rays;
Interleukin-1beta/metabolism;
Lipopolysaccharides/pharmacology;
Mice;
Nitric Oxide/metabolism;
Nitric Oxide Synthase Type II/metabolism
- From:The Korean Journal of Laboratory Medicine
2009;29(4):338-344
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Near-infrared light (NIR, 0.8-1.5 micrometer light) has been used in therapeutic devices for various injuries such as infected, ischemic and hypoxic wound. NIR-emitting technology has been developed recently in Korea. We hypothesized that NIR may have an anti-inflammatory effect and investigated the effect of NIR-irradiated media on cell culture. METHODS: Three kinds of cell lines, CAPE (vascular endothelial cell), NIH3T3 (fibroblast), and RD (smooth muscle cell) cells were cultured for 4 days in 10% FBS-containing media (1x10(4) cells/well), which were irradiated or not irradiated (control) by Eco-NFIR Drive (Model #0210, Ecowavetech, Korea). The cells were stimulated by 10 mcg/mL of bacterial lipopolysaccharide (LPS) or sodium nitroprusside (SNP). Cellular proliferation was measured by methylthiazol tetrazolium assay. Expression of interleukin (IL)-1 beta and nitric oxide was measured by ELISA. Expression of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) was measured by immunofluorescence staining. RESULTS: NIR-irradiated medium was favorable for CAPE cell proliferation (N=8, P=0.000). IL-1 beta secretion from LPS-stimulated NIH3T3 cells incubated in the NIR medium was below that of control medium (N=4, P=0.026). Nitrate production seemed to be low in NIR-irradiated medium although statistically insignificant (N=4, P=0.076). Expression of iNOS of the LPS-stimulated cells was decreased in NIR medium, however, Cox-2 expression was not different between the two media. CONCLUSIONS: NIR-irradiated medium supported vascular endothelial cell proliferation and showed an anti-inflammatory effect on fibroblast culture. These results can be used as basic data for future research on the clinical application of NIR.
