Effect of Chinese herbal compound "Jisuikang" on engulfment of neuron debris by microglia
10.3969/j.issn.1006-5725.2017.20.007
- VernacularTitle:中药复方脊髓康对小胶质细胞吞噬神经元碎片作用的影响
- Author:
Wenchao YUAN
1
;
Lei WANG
;
Yong MA
;
Guicheng HUANG
;
Longyun ZHOU
;
Yang GUO
Author Information
1. 210023,南京中医药大学
- Keywords:
Chinese herbal compound;
microglia;
primary neurons;
phagocytosis;
transinfection
- From:
The Journal of Practical Medicine
2017;33(20):3359-3363
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Chinese herbal compound"Jisuikang"on the phagocyto-sis of neuronal debris by microglial cells. Methods To prepare serum containing drugs of JSK and divide them into the low,middle and high dose groups,the blank serum group and LPS+blank serum group. BV2 was labeled by lentiviral vectors containing the green fluorescent protein gene (GFP). To establish the damage neuron model and mix injured neurons with the transfected microglia. To observe the situation of microglia which was affected by serum containing drugs devour the neuronal debris. Results The middle and high dose of JSK showed greater phagocytic percentage and phagocytic index than those of the control group(P<0.001). In comparison of LPS+blank serum group,no significant difference was found in the middle and high dose of JSK. However,to the phagocytic index, which was better than that of LPS+blank serum group(P<0.05). Conclusion JSK may enhance the engulfment of neuron debris by BV2,which could provide a better living environment for the growth of neurons.
