The effects of curcumin on myosin light chain kinase in the intestinal mucosa of rats with nonalcoholic fat-ty liver disease
10.3969/j.issn.1006-5725.2017.20.008
- VernacularTitle:姜黄素对非酒精性脂肪肝大鼠肠黏膜肌球蛋白轻链激酶的影响
- Author:
Hongtao HOU
1
;
Yanmei QIU
;
Jian ZHANG
;
Yiting HU
;
Yun BAI
;
Cunkai WANG
;
Jimin ZHENG
Author Information
1. 河北省人民医院消化一科
- Keywords:
nonalcoholic fatty liver;
curcumin;
myosin light chain kinase;
intestinal mucosa
- From:
The Journal of Practical Medicine
2017;33(20):3363-3367
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of curcumin on myosin light chain kinase(MLCK)in the intestinal mucosa of rats with nonalcoholic fatty liver disease(NAFLD). Methods 35 SD rats were randomly di-vided into the normal control group ,the high fat group and the curcumin group. The high fat group ,curcumin group were given high fat diet for 16 weeks. After 8 weeks of high fat feeding in the curcumin group ,the rats were gavaged with 200 mg/(kg·d)curcumin for 8 weeks. The levels of ALT,AST,LPS and DAO in blood and expres-sion of MLCK in the intestinal mucosa were detected. The changes of liver pathology and tight junction(TJ)of the intestinal mucosa were observed. Results Compared with the control group,the blood levels of ALT,AST,LPS and DAO in the high fat group were obviously increased(P<0.05);Compared with the high fat group,the blood levels of ALT、AST、LPS and DAO in the curcumin group were obviously decreased (P < 0.05). In the high fat group ,hepatocellular steatosis was obvious ,while in the curcumin group hepatocellular steatosis was decreased. TJ was disrupted in the high fat group ,and the intercellular space was larger in the TJ group than the control group (P<0.05). The intercellular space was narrower in the curcumin group than the high fat group(P<0.05). The expression of MLCK in the high fat group was significant higher than that in the control group(P<0.05). The pos-itive staining in the curcumin group was significant lower than that in the high fat group (P < 0.05). Conclusion Curcumin can ameliorate hepatic steatosis by downregulating expression of MLCK in the intestinal mucosa of rats with NAFLD,improving TJ structure of the intestinal mucosa.
